Our research can be broken down into two broad areas. These relate to the basic understanding of developmental processes and how stem cells can be used for therapeutic purposes. Understanding fundamental processes of development will give insight into how cells are specified and assembled into different organs. Understanding this process is crucial if we are to find cures for disease states. We are applying our understanding of developmental biology to the differentiation of human ESCs into pancreatic cells that can be used for transplantation into diabetic patients. Together, we feel our research goals are well aligned with the Institute's mission.
Our work is directed towards defining early developmental processes so that a more thorough understanding of human disease states can be obtained. Through a detailed understanding of early development, we hope to apply this information to develop approaches for the differentiation of embryonic stem cells into therapeutically useful cell types that can be used to cure degenerative diseases and treat acute injury.
White, J. and Dalton, S. (2005). Cell cycle control of embryonic stem cells. Stem Cell Rev. 1, 131-138. Dalton, S. (2006). It’s endoderm…definitively! Regenerative Med. 1, 381-383.
McLean, M., D'Amour, K., Krishnamoorthy, M., Kulik, M., Reynolds, D., Sheppard, A., Baetge, E. and Dalton, S. (2007). Activin A efficiently specifies definitive endoderm from human embryonic stem cells only when PI3K signaling is suppressed. Stem Cells 25, 29-38.
Nash, R., Neves, L., Faast, R. Pierce, M. and Dalton, S. (2007). The lectin DBA recognizes glycan epitopes on the surface of murine embryonic stem cells: a new tool for characterizing pluripotent cells and early differentiation. Stem Cells 25, 974-982.
Liu, H., Dalton, S. and Xu, Y. (2007). Transcriptional profiling of definitive endoderm derived from human embryonic stem cells. Comput Syst Bioinformatics Conf. 6: 79-82.
Nairn, A.V., Kinoshita-Toyoda, A., Toyoda, H., Xie, J., Harris, K., Dalton, S., Kulik, M., Pierce, J.M., Toida, T., Moremen, K.W., Linhardt, R.J. (2007). Glycomics of proteoglycan biosynthesis in murine embryonic stem cell differentiation. J Proteome Res. 6: 4374-4387.
Ohtsuka, S. and Dalton, S. (2008). The biology of embryonic pluripotency. Gene Therapy 15: 74-81. Dalton, S. (2008). Cardiac stem cells: at the heart of cell therapy. Regenerative Med. 3: 381-388. Dalton, S. (2009). Exposing hidden dimensions of embryonic stem cell cycle control. Cell Stem Cells 9-10.
Bechard, S. and Dalton, S. (2009). PI3K/AKT1 and GSK3beta control embryonic stem cell fate decisions by a novel mechanism converging on c-myc". Molecular and Cellular Biology, in press.